- Several stem cell mobilization strategies have been employed in the past 2 decades, including chemotherapy, hematopoietic growth factors, and chemotherapy plus growth factors. Granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage CSF are standard agents approved for peripheral blood stem cell mobilization since the early 1990s. Between 5% and 20% of patients, however, fail to mobilize a sufficient numbers of peripheral blood stem cells in response to G-CSF with or without chemotherapy.
- To fulfill the dual abilities to self-renew and to differentiate into cells of multiple lineages, stem cells must undergo, at some stage, asymmetric divisions to generate cells to sustain the stem cell pool as well as the various progeny cells of the distinct lineages. A central question in developmental biology is how a single cell can divide to produce two progeny cells that adopt different fates. Different daughter cells can theoretically arise by uneven distribution of determinants upon cell division, i.e., due to intrinsic factors, or become different upon subsequent exposure to environmental signals, i.e., due to extrinsic factors.
- This annual symposium, held alternately in San Diego and Heidelberg, has remained a forum for exchange of innovative ideas among scientists and clinicians from academic institutions as well as from the biomedical industry. As we initiated this symposium at the University of California, San Diego, in March 1992, the clinical use of progenitor cells derived from peripheral blood instead of the marrow was seen with skepticism and regarded as experimental. In the meantime, the role of blood-derived progenitor cells is well established.