Experimental Hematology
Volume 38, Issue 10 , Pages 945-956 , October 2010

Multipotent mesenchymal stem cell grafting to treat cutaneous radiation syndrome: Development of a new minipig model

  • Diane Agay

      Affiliations

    • IRBA-antenne La Tronche-CRSSA, La Tronche, France
  • ,
  • Harry Scherthan

      Affiliations

    • Institut für Radiobiologie der Bundeswehr in Verbindung mit der Universität Ulm, München, Germany
  • ,
  • Fabien Forcheron

      Affiliations

    • IRBA-antenne La Tronche-CRSSA, La Tronche, France
  • ,
  • Nancy Grenier

      Affiliations

    • IRBA-antenne La Tronche-CRSSA, La Tronche, France
  • ,
  • Francis Hérodin

      Affiliations

    • IRBA-antenne La Tronche-CRSSA, La Tronche, France
  • ,
  • Viktor Meineke

      Affiliations

    • Institut für Radiobiologie der Bundeswehr in Verbindung mit der Universität Ulm, München, Germany
  • ,
  • Michel Drouet

      Affiliations

    • IRBA-antenne La Tronche-CRSSA, La Tronche, France
    • Corresponding Author InformationOffprint requests to: Michel Drouet, Ph.D., M.D., IRBA-antenne La Tronche-CRSSA, 24 Avenue des Maquis du Grésivaudan, La Tronche 38702, France

Received 13 January 2010 ,Revised 10 June 2010 ,Accepted 21 June 2010.

References 

  1. Hopewell JW. The skin: its structure and response to ionizing radiation. Int J Radiat Biol. 1990;57:751–773
  2. Lefaix JL, Delanian S. Le syndrome cutané radio-induit. In:  de Revel T,  Gourmelon P,  Vidal D,  Renaudeau C editor. The terrorist threat nuclear, radiological, biological, chemical—a medical approach. Montrouge: John Libbey Eurotext; 2005;p. 87–95
  3. Peter RU. The cutaneous radiation syndrome. In:  MacVittie TJ,  Weiss JF,  Browne D editor. Advances in the treatment of radiation injuries. Oxford: Elsevier Sciences Ltd; 1996;p. 237–240
  4. Peter RU. Management of the skin injuries in radiation accidents. In:  Ricks RC,  Berger ME,  O’Hara FM editor. The cutaneous radiation syndrome. The clinical care of victims. London: The Parthenon Publishing Group; 2001;p. 225–229
  5. Fliedner TM, Dörr HD, Meineke V. Multi-organ involvement as pathogenetic principle of the radiation syndromes: a study involving 110 case histories documented in SEARCH and classified as the bases of haematopoietic indicators of effect. BJR Suppl. 2005;27:1–8
  6. Peter RU. Cutaneous radiation syndrome in multi-organ failure. BJR Suppl. 2005;27:180–184
  7. Stephanazzi J, Bargues L, Curet PM, et al. Le traitement du syndrome cutané radiologique. In:  de Revel T,  Gourmelon P,  Vidal D,  Renaudeau C editor. The terrorist threat nuclear, radiological, biological, chemical—a medical approach. Montrouge: John Libbey Eurotext; 2005;p. 112–122
  8. Lataillade JJ, Doucet C, Bey E, et al. New approach to radiation burn treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy. Regen Med. 2007;2:785–794
  9. François S, Mouissedine M, Mathieu N, et al. Human mesenchymal stem cells favour healing of the cutaneous radiation syndrome in a xenogenic transplant model. Ann Hematol. 2007;86:1–8
  10. François S, Bensidhoum M, Mouiseddine M, et al. Local irradiation induces not only homing of human mesenchymal stem cells (huMSC) at exposed sites but promotes their widespread engraftment to multiple organs: a study of their quantitative distribution following irradiation damages. Stem Cells. 2006;24:1020–1029
  11. Phinney DG, Prockop DJ. Mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair-current views. Stem Cells. 2007;25:2896–2902
  12. Chamberlain G, Fox J, Ashton B, Middleton J. Mesenchymal stem cells: their phenotype, differentiation capacity, immunological features and potential for homing. Stem Cells. 2008;25:2739–2749
  13. Miura M, Miura Y, Padilla-Nash HM, et al. Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation. Stem Cells. 2006;24:1095–1103
  14. Tarte K, Gaillard J, Lataillade JJ, et al. Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation. Blood. 2010;115:1549–1553
  15. Archambeau JO, Bennett GW, Abata JJ, Brenneis HJ. Response of swine skin microvasculature to acute single exposures of x rays: quantification of the epidermal cell changes. Radiat Res. 1979;79:298–337
  16. Archambeau JO, Ines A, Fajardo LF. Response of swine skin microvasculature to acute single exposures of X rays: quantification of endothelial changes. Radiat Res. 1984;98:37–51
  17. Bey E, Prat M, Duhamel P, et al. Emerging therapy for improving wound repair of severe radiation burns using local bone marrow-derived stem cell administrations. Wound Repair Regen. 2010;18:50–58
  18. Scherthan H, Hieber L, Braselmann H, et al. Accumulation of DSBs in gamma-H2AX domains fuel chromosomal aberrations. Biochem Biophys Res Commun. 2008;371:694–697
  19. Vodicka P, Smetana K, Dvorankova B, et al. The miniature pig as an animal model in biomedical research. Ann NY Acad Sci. 2005;1049:161–171
  20. Lefaix JL, Delanian S, Leplat JJ, et al. Successful treatment of radiation-induced fibrosis using Cu/Zn-SOD and Mn-SOD: an experimental study. Int J Radiat Oncol Biol Phys. 1996;35:305–312
  21. Delanian S, Porcher R, Balla-Mekias S, et al. Randomized, placebo-controlled trial of combined pentoxifylline and tocopherol for regression of superficial radiation-induced fibrosis. J Clin Oncol. 2003;21:2545–2550
  22. Martin M, Lefaix JL, Pinton P, et al. Temporal modulation of TGF-beta 1 and beta-actin gene expression in pig skin and muscular fibrosis after ionizing radiation. Radiat Res. 1993;134:63–70
  23. Swindle MM, Smith AC. Comparative anatomy and physiology of the pig. Scand J Lab Amin Sci. 1998;25(Suppl 1):11–21
  24. Mahl JA, Vogel BE, Court M, et al. The minipig in dermatotoxicology: methods and challenges. Exp Toxicol. Pathol. 2006;57:341–345
  25. Van den Aardweg GJMJ, Arnold M, Hopewell JW. A comparison of the radiation response of the epidermidis in two strains of pig. Radiat Res. 1990;124:283–287
  26. Löbrich M, Rief N, Kühne M, et al. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci U S A. 2005;102:8984–8989
  27. Chen L, Tredget EE, Wu PY, Wu Y. Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS One. 2008;3:e1886
  28. Rehman J, Traktuev D, Jingling L, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004;109:1292–1298
  29. Thangarajah H, Vial IN, Chang E, et al. IFATS collection: adipose stromal cells adopt a proangiogenic phenotype under the influence of hypoxia. Stem Cells. 2009;27:266–274
  30. Sasaki M, Abe Y, Ando S, et al. Mesenchymal stem cells are recruited into wounded skin and contribute to wound repair by transdifferentiation into multiple skin cell type. J Immunol. 2008;180:2581–2587
  31. Wu Y, Chen L, Scott PG, et al. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells. 2007;25:2648–2659
  32. Shi C, Zhu Y, Su Y, et al. Stem cells and their applications in skin-cell therapy. Trends Biotechnol. 2006;24:48–52

PII: S0301-472X(10)00243-2

doi: 10.1016/j.exphem.2010.06.008

Experimental Hematology
Volume 38, Issue 10 , Pages 945-956 , October 2010