Experimental Hematology
Volume 38, Issue 9 , Pages 809-818.e2 , September 2010

Induction of pluripotency in human cord blood unrestricted somatic stem cells

  • Holm Zaehres

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Gesine Kögler

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Marcos J. Arauzo-Bravo

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Martina Bleidissel

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Simeon Santourlidis

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Sandra Weinhold

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Boris Greber

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Jeong Beom Kim

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Anja Buchheiser

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Stefanie Liedtke

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Hanna M. Eilken

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Nina Graffmann

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Xiaoyi Zhao

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Johann Meyer

      Affiliations

    • Hannover Medical School, Cluster of Excellence “REBIRTH,” Hannover, Germany
  • ,
  • Peter Reinhardt

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Boris Burr

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Simon Waclawczyk

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Claudia Ortmeier

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
  • ,
  • Markus Uhrberg

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
  • ,
  • Hans R. Schöler

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
    • Hannover Medical School, Cluster of Excellence “REBIRTH,” Hannover, Germany
    • Drs. Schöler, Cantz, and Wernet contributed equally to this work.
  • ,
  • Tobias Cantz

      Affiliations

    • Max Planck Institute for Molecular Biomedicine, Münster, Germany
    • Hannover Medical School, Cluster of Excellence “REBIRTH,” Hannover, Germany
    • Drs. Schöler, Cantz, and Wernet contributed equally to this work.
  • ,
  • Peter Wernet

      Affiliations

    • Heinrich-Heine-University Düsseldorf, Institute for Transplantation Diagnostics and Cell Therapeutics, Düsseldorf, Germany
    • Corresponding Author InformationOffprint requests to: Peter Wernet, M.D., Ph.D., Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, NRW, Germany
    • Drs. Schöler, Cantz, and Wernet contributed equally to this work.

Received 3 February 2010 ,Revised 30 April 2010 ,Accepted 10 May 2010.

References 

  1. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861–872
  2. Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917–1920
  3. Park IH, Zhao R, West JA, et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008;451:141–146
  4. Masaki H, Ishikawa T, Takahashi S, et al. Heterogeneity of pluripotent marker gene expression in colonies generated in human iPS cell induction culture. Stem Cell Res. 2007;1:105–115
  5. Lowry WE, Richter L, Yachechko R, et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008;105:2883–2888
  6. Huangfu D, Osafune K, Maehr R, et al. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol. 2008;26:1269–1275
  7. Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R. A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008;3:346–353
  8. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008;3:340–345
  9. Dimos JT, Rodolfa KT, Niakan KK, et al. Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science. 2008;321:1218–1221
  10. Aasen T, Raya A, Barrero MJ, et al. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008;26:1276–1284
  11. Loh YH, Agarwal S, Park IH, et al. Generation of induced pluripotent stem cells from human blood. Blood. 2009;113:5476–5479
  12. Kim JB, Greber B, Arauzo-Bravo MJ, et al. Direct reprogramming of human neural stem cells by OCT4. Nature. 2009;461:649–643
  13. Kögler G, Sensken S, Airey JA, et al. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004;200:123–135
  14. Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005;122:947–956
  15. Zaehres H, Daley GQ. Transgene expression and RNA interference in embryonic stem cells. Methods Enzymol. 2006;420:49–64
  16. Nakano T, Kodama H, Honjo T. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science. 1994;265:1098–1101
  17. Choi KD, Yu J, Smuga-Otto K, et al. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells. 2009;27:559–567
  18. Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC. MicroRNA profiling of human-induced pluripotent stem cells. Stem Cells Dev. 2009;18:749–758
  19. Yu J, Hu K, Smuga-Otto K, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797–801
  20. Eminli S, Foudi A, Stadtfeld M, et al. Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells. Nat Genet. 2009;41:968–976
  21. Ye Z, Zhan H, Mali P, et al. Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders. Blood. 2009;114:5473–5480
  22. Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S. Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53. Exp Hematol. 38:154–162.
  23. Haase A, Olmer R, Schwanke K, et al. Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell. 2009;5:434–441
  24. Giorgetti A, Montserrat N, Aasen T, et al. Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2. Cell Stem Cell. 2009;5:353–357
  25. Raya A, Rodriguez-Piza I, Guenechea G, et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009;460:53–59
  26. Lengerke C, Grauer M, Niebuhr NI, et al. Hematopoietic development from human induced pluripotent stem cells. Ann N Y Acad Sci. 2009;1176:219–227
  27. Grigoriadis AE, Kennedy M, Bozec A, et al. Directed differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells. Blood. 115:2769–2776.
  28. Feng Q, Lu SJ, Klimanskaya I, et al. Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence. Stem Cells. 2010;28:704–712
  29. Kim D, Kim CH, Moon JI, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. 2009;4:472–476
  30. Shi Y, Do JT, Desponts C, Hahm HS, Schöler HR, Ding S. A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell. 2008;2:525–528

PII: S0301-472X(10)00236-5

doi: 10.1016/j.exphem.2010.05.009

Experimental Hematology
Volume 38, Issue 9 , Pages 809-818.e2 , September 2010