Experimental Hematology
Volume 38, Issue 9 , Pages 792-797 , September 2010

Of mice and men: Human RNA polymerase III promoter U6 is more efficient than its murine homologue for shRNA expression from a lentiviral vector in both human and murine progenitor cells

Received 22 April 2010 ,Revised 27 April 2010 ,Accepted 17 May 2010.

References 

  1. Brenz Verca M, Weber P, Mayer C, et al. Development of a species-specific RNA polymerase I-based shRNA expression vector. Nucleic Acids Res. 2007;35:e10;Epub 2006 Dec 7
  2. Sandy P, Ventura A, Jacks T. Mammalian RNAi: a practical guide. Biotechniques. 2005;39:215–224
  3. Meister G, Tuschl T. Mechanisms of gene silencing by double-stranded RNA. Nature. 2004;431:343–349
  4. Dykxhoorn DM, Novina CD, Sharp PA. Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol. 2003;4:457–467
  5. Das G, Henning D, Reddy R. Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes. J Biol Chem. 1987;262:1187–1193
  6. Zenke K, Kim KH. Novel fugu U6 promoter driven shRNA expression vector for efficient vector based RNAi in fish cell lines. Biochem Biophys Res Commun. 2008;371:480–483
  7. Lambeth LS, Wise TG, Moore RJ, Muralitharan MS, Doran TJ. Comparison of bovine RNA polymerase III promoters for short hairpin RNA expression. Anim Genet. 2006;37:369–372
  8. Wise TG, Schafer DJ, Lambeth LS, et al. Characterization and comparison of chicken U6 promoters for the expression of short hairpin RNAs. Anim Biotechnol. 2007;18:153–162
  9. Weber K, Bartsch U, Stocking C, Fehse B. A multicolor panel of novel lentiviral “gene ontology” (LeGO) vectors for functional gene analysis. Mol Ther. 2008;16:698–706
  10. Mutschler M, Magin AS, Buerge M, et al. NF-E2 overexpression delays erythroid maturation and increases erythrocyte production. Br J Haematol. 2009;146:203–217
  11. Goerttler PS, Kreutz C, Donauer J, et al. Gene expression profiling in polycythaemia vera: overexpression of transcription factor NF-E2. Br J Haematol. 2005;129:138–150

PII: S0301-472X(10)00201-8

doi: 10.1016/j.exphem.2010.05.005

Experimental Hematology
Volume 38, Issue 9 , Pages 792-797 , September 2010

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