Derivation of multipotent progenitors from human circulating CD14⁺ monocytes

Morphology of monocyte-derived multipotential cells (MOMCs), macrophages, and dendritic cells, which are obtained by culturing peripheral blood CD14⁺ monocytes. Original magnification: ×80.

Effect of monocyte-derived multipotential cells (MOMCs) on ex vivo expansion of cord blood CD34⁺ cells. One-thousand human cord blood CD34⁺ cells were plated on a monolayer of MOMCs, bone marrow stroma cells, or macrophages without any exogenous growth factors. (A) After 1 and 2 weeks of cultures, cells were harvested and the number of total nucleated cells was counted. Results shown are the mean and standard deviation of eight independent experiments. (B) Recovered cells were subjected to colony-forming cell assay for evaluating burst-forming unit erythroid (BFU-E), colony-forming unit granulocyte-macrophage (CFU-GM), and CFU granulocyte-erythrocyte-macrophage-megakaryocyte (CFU-GEMM). Results shown are the mean of four independent experiments.