Experimental Hematology
Volume 38, Issue 6 , Pages 446-452 , June 2010

Design of Ad5F35 vectors for coordinated dual gene expression in candidate human hematopoietic stem cells

  • Manli Na

      Affiliations

    • The Rausing Laboratory, Lund University, Lund, Sweden
    • ,
  • Xiaolong Fan

      Affiliations

    • The Rausing Laboratory, Lund University, Lund, Sweden
    • College of Life Sciences, Beijing Normal University, Beijing, China
    • Corresponding Author InformationOffprint requests to: Xiaolong Fan, M.D., Ph.D., The Rausing Laboratory, BMC D14 SE-221 84 Lund, Sweden

Received 21 January 2010 ,Revised 24 February 2010 ,Accepted 3 March 2010.

References 

  1. Fan X, Brun A, Karlsson S. Adenoviral vector design for high-level transgene expression in primitive human hemtopoietic progenitors. Gene Ther. 2000;7:2132–2138
  2. Jaras M, Brun AC, Karlsson S, Fan X. Adenoviral vectors for transient gene expression in human primitive hematopoietic cells: applications and prospects. Exp Hematol. 2007;35:343–349
  3. Fan X, Valdimarsdottir G, Larsson J, et al. Transient disruption of autocrine TGF-beta signaling leads to enhanced survival and proliferation potential in single primitive human hemopoietic progenitor cells. J Immunol. 2002;168:755–762
  4. Jaras M, Edqvist A, Rebetz J, Salford LG, Widegren B, Fan X. Human short-term repopulating cells have enhanced telomerase reverse transcriptase expression. Blood. 2006;108:1084–1091
  5. Nilsson M, Karlsson S, Fan X. Functionally distinct subpopulations of cord blood CD34+ cells are transduced by adenoviral vectors with serotype 5 or 35 tropism. Mol Ther. 2004;9:377–388
  6. Nilsson M, Ljungberg J, Richter J, et al. Development of an adenoviral vector system with adenovirus serotype 35 tropism; efficient transient gene transfer into primary malignant hematopoietic cells. J Gene Med. 2004;6:631–641
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  9. Mizuguchi H, Xu Z, Ishii-Watabe A, Uchida E, Hayakawa T. IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther. 2000;1:376–382
  10. Amendola M, Venneri MA, Biffi A, Vigna E, Naldini L. Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters. Nat Biotech. 2005;23:108–116
  11. Maetzig T, Galla M, Brugman MH, Loew R, Baum C, Schambach A. Mechanisms controlling titer and expression of bidirectional lentiviral and gammaretroviral vectors. Gene Ther. 2010;17:400–411
  12. Mittereder N, March KL, Trapnell BC. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J Virol. 1996;70:7498–7509
  13. Hogan CJ, Shpall EJ, McNulty O, et al. Engraftment and development of human CD34(+)-enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice. Blood. 1997;90:85–96
  14. Rossi MI, Medina KL, Garrett K, et al. Relatively normal human lymphopoiesis but rapid turnover of newly formed B cells in transplanted nonobese diabetic/SCID mice. J Immunol. 2001;167:3033–3042
  15. Edqvist A, Rebetz J, Jaras M, et al. Detection of cell cycle- and differentiation stage-dependent human telomerase reverse transcriptase expression in single living cancer cells. Mol Ther. 2006;14:139–148
  16. Fan X, Brun A, Segren S, Jacobsen SE, Karlsson S. Efficient adenoviral vector transduction of human hematopoietic SCID-repopulating and long-term culture-initiating cells. Hum Gene Ther. 2000;11:1313–1327

PII: S0301-472X(10)00098-6

doi: 10.1016/j.exphem.2010.03.007

Experimental Hematology
Volume 38, Issue 6 , Pages 446-452 , June 2010

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