Fifth complement cascade protein (C5) cleavage fragments disrupt the SDF-1/CXCR4 axis: Further evidence that innate immunity orchestrates the mobilization of hematopoietic stem/progenitor cells

Jalili, Ali; Shirvaikar, Neeta; Marquez-Curtis, Leah; Qiu, Yuanyuan; Korol, Chris; Lee, HakMo; Turner, A. Robert; Ratajczak, Mariusz Z.; Janowska-Wieczorek, Anna

doi:10.1016/j.exphem.2010.02.002

« Previous Next »Experimental Hematology
Volume 38, Issue 4 , Pages 321-332, April 2010

Fifth complement cascade protein (C5) cleavage fragments disrupt the SDF-1/CXCR4 axis: Further evidence that innate immunity orchestrates the mobilization of hematopoietic stem/progenitor cells

Ali Jalili
- Canadian Blood Services R & D, University of Alberta, Edmonton, Alberta, Canada
- Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
Neeta Shirvaikar
- Canadian Blood Services R & D, University of Alberta, Edmonton, Alberta, Canada
- Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
Leah Marquez-Curtis
- Canadian Blood Services R & D, University of Alberta, Edmonton, Alberta, Canada
Yuanyuan Qiu
- Canadian Blood Services R & D, University of Alberta, Edmonton, Alberta, Canada
Chris Korol
- Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
HakMo Lee
- University of Louisville, Louisville, Ky., USA
A. Robert Turner
- Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
Mariusz Z. Ratajczak
- University of Louisville, Louisville, Ky., USA
Anna Janowska-Wieczorek
- Canadian Blood Services R & D, University of Alberta, Edmonton, Alberta, Canada
- Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
- Offprint requests to: Anna Janowska-Wieczorek, M.D., Ph.D., Department of Medicine, University of Alberta, CBS Edmonton Centre, 8249-114 Street NW, Edmonton, Alberta T6G 2R8, Canada

Received 18 September 2009; received in revised form 15 January 2010; accepted 5 February 2010. published online 15 February 2010.

Objective

Having previously demonstrated that the complement system modulates mobilization of hematopoietic stem/progenitor cells (HSPC) in mice, we investigated the involvement of C5 cleavage fragments (C5a/_desArgC5a) in human HSPC mobilization.

Materials and Methods

C5 cleavage fragments in the plasma were evaluated by enzyme-linked immunosorbent assay using human anti-_desArgC5a antibody, and expression of the C5a/_desArgC5a receptor (CD88) in hematopoietic cells by flow cytometry. We also examined the chemotactic responses of hematopoietic cells to C5 cleavage fragments and expression of stromal cell−derived factor-1 (SDF-1)−degrading proteases that perturb retention of HSPC in bone marrow, namely matrix metalloproteinase (MMP)-9, membrane type (MT) 1−MMP, and carboxypeptidase M.

Results

We found that plasma levels of _desArgC5a are significantly higher in patients who are good mobilizers and correlate with CD34⁺ cell and white blood cell counts in mobilized peripheral blood. C5 cleavage fragments did not chemoattract myeloid progenitors (colony-forming unit granulocyte-macrophage), but _desArgC5a did strongly chemoattract mature nucleated cells. Consistently, CD88 was not detected on CD34⁺ cells, but appeared on more mature myeloid precursors, monocytes, and granulocytes. Moreover, granulocyte colony-stimulating factor−mobilized peripheral blood mononuclear cells and polymorphonuclear cells had a significantly higher percentage of cells expressing CD88 than nonmobilized peripheral blood. Furthermore, C5a stimulation of granulocytes and monocytes decreased CXCR4 expression and chemotaxis toward an SDF-1 gradient and increased secretion of MMP-9 and expression of MT1−MMP and carboxypeptidase M.

Conclusion

C5 cleavage fragments not only induce a highly proteolytic microenvironment in human bone marrow, which perturbs retention through the CXCR4/SDF-1 axis, but also strongly chemoattracts granulocytes, promoting their egress into mobilized peripheral blood, which is crucial for subsequent mobilization of HSPC.