Experimental Hematology
Volume 38, Issue 1 , Pages 3-10 , January 2010

Successful engraftment by leukemia initiating cells in adult acute lymphoblastic leukemia after direct intrahepatic injection into unconditioned newborn NOD/SCID mice

  • Alice M.S. Cheung

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • Drs. Cheung and Fung contributed equally to this work.
    • ,
  • Tsz-Kan Fung

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • Drs. Cheung and Fung contributed equally to this work.
    • ,
  • August K.P. Fan

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Thomas S.K. Wan

      Affiliations

    • Department of Pathology, The University of Hong Kong, Hong Kong
    • ,
  • Howard C.H. Chow

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Joseph C.K. Leung

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Loretta Y.Y. Chan

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Yok-Lam Kwong

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Raymond Liang

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Anskar Y.H. Leung

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • Corresponding Author InformationOffprint requests to: Anskar Y.H. Leung, M.D., Ph.D., Department of Medicine, Queen Mary Hospital, Pok Fu Lam Road, Room K418, K Block, Hong Kong

Received 4 August 2009 ,Revised 10 October 2009 ,Accepted 14 October 2009.

References 

  1. Pui CH. Acute lymphoblastic leukemia in children. Curr Opin Oncol. 2000;12:3–12
  2. Gokbuget N, Hoelzer D. Treatment of adult acute lymphoblastic leukemia. Semin Hematol. 2009;46:64–75
  3. Bernt KM, Armstrong SA. Leukemia stem cells and human acute lymphoblastic leukemia. Semin Hematol. 2009;46:33–38
  4. Lapidot T, Fajerman Y, Kollet O. Immune-deficient SCID and NOD/SCID mice models as functional assays for studying normal and malignant human hematopoiesis. J Mol Med. 1997;75:664–673
  5. Ishikawa F, Livingston AG, Wingard JR, Nishikawa S, Ogawa M. An assay for long-term engrafting human hematopoietic cells based on newborn NOD/SCID/beta2-microglobulin(null) mice. Exp Hematol. 2002;30:488–494
  6. Ishikawa F, Yasukawa M, Lyons B, et al. Development of functional human blood and immune systems in NOD/SCID/IL2 receptor {gamma} chain(null) mice. Blood. 2005;106:1565–1573
  7. McKenzie JL, Gan OI, Doedens M, Dick JE. Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells. Blood. 2005;106:1259–1261
  8. Ishikawa F, Yoshida S, Saito Y, et al. Chemotherapy-resistant human AML stem cells home to and engraft within the bone-marrow endosteal region. Nat Biotechnol. 2007;25:1315–1321
  9. Traggiai E, Chicha L, Mazzucchelli L, et al. Development of a human adaptive immune system in cord blood cell-transplanted mice. Science. 2004;304:104–107
  10. Robert-Richard E, Ged C, Ortet J, et al. Human cell engraftment after busulfan or irradiation conditioning of NOD/SCID mice. Haematologica. 2006;91:1384
  11. Cheung AM, Wan TS, Leung JC, et al. Aldehyde dehydrogenase activity in leukemic blasts defines a subgroup of acute myeloid leukemia with adverse prognosis and superior NOD/SCID engrafting potential. Leukemia. 2007;21:1423–1430
  12. Cheung AM, Chow HC, Liang R, Leung AY. A comparative study of bone marrow and peripheral blood CD34+ myeloblasts in acute myeloid leukaemia. Br J Haematol. 2009;144:484–491
  13. Reynaud D, Lefort N, Manie E, Coulombel L, Levy Y. In vitro identification of human pro-B cells that give rise to macrophages, natural killer cells, and T cells. Blood. 2003;101:4313–4321
  14. Cox CV, Evely RS, Oakhill A, Pamphilon DH, Goulden NJ, Blair A. Characterization of acute lymphoblastic leukemia progenitor cells. Blood. 2004;104:2919–2925
  15. le Viseur C, Hotfilder M, Bomken S, et al. In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties. Cancer Cell. 2008;14:47–58
  16. Cheung AM, Chow HC, Kwong YL, Liang R, Leung AY. FLT3/internal tandem duplication subclones in acute myeloid leukemia differ in their engraftment potential in NOD/SCID mice. Leuk Res. 2009 Aug 14;[Epub ahead of print]
  17. Mullighan CG, Phillips LA, Su X, et al. Genomic analysis of the clonal origins of relapsed acute lymphoblastic leukemia. Science. 2008;322:1377–1380
  18. Cox CV, Blair A. A primitive cell origin for B-cell precursor ALL?. Stem Cell Rev. 2005;1:189–196
  19. Castor A, Nilsson L, Astrand-Grundstrom I, et al. Distinct patterns of hematopoietic stem cell involvement in acute lymphoblastic leukemia. Nat Med. 2005;11:630–637

PII: S0301-472X(09)00398-1

doi: 10.1016/j.exphem.2009.10.007

Experimental Hematology
Volume 38, Issue 1 , Pages 3-10 , January 2010

Article Tools

* Image Usage & Resolution