Experimental Hematology
Volume 37, Issue 12 , Pages 1379-1386.e4 , December 2009

A novel zebrafish jak2aV581F model shared features of human JAK2V617F polycythemia vera

  • Alvin C.H. Ma

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • August Fan

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Alister C. Ward

      Affiliations

    • School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia
    • ,
  • Clifford Liongue

      Affiliations

    • School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia
    • ,
  • Rowena S. Lewis

      Affiliations

    • School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia
    • ,
  • Suk H. Cheng

      Affiliations

    • Department of Biology and Chemistry, City University of Hong Kong, Hong Kong
    • ,
  • P.K. Chan

      Affiliations

    • Department of Biology and Chemistry, City University of Hong Kong, Hong Kong
    • ,
  • Sze-Fai Yip

      Affiliations

    • Department of Pathology, The University of Hong Kong, Hong Kong
    • ,
  • Raymond Liang

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • ,
  • Anskar Y.H. Leung

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong
    • Corresponding Author InformationOffprint requests to: Anskar Y.H. Leung, M.D., Ph.D., Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Pok Fu Lam Road, Room K418, K Block, Hong Kong

Received 27 June 2009 ,Revised 28 August 2009 ,Accepted 31 August 2009.

References 

  1. Lensch MW, Daley GQ. Origins of mammalian hematopoiesis: in vivo paradigms and in vitro models. Curr Top Dev Biol. 2004;60:127–196
  2. Carradice D, Lieschke GJ. Zebrafish in hematology: sushi or science?. Blood. 2008;111:3331–3342
  3. Davidson AJ, Zon LI. The ‘definitive’ (and ‘primitive’) guide to zebrafish hematopoiesis. Oncogene. 2004;23:7233–7246
  4. Lieschke GJ, Oates AC, Paw BH, et al. Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning. Dev Biol. 2002;246:274–295
  5. Onnebo SM, Yoong SH, Ward AC. Harnessing zebrafish for the study of white blood cell development and its perturbation. Exp Hematol. 2004;32:789–796
  6. Herbomel P, Thisse B, Thisse C. Ontogeny and behaviour of early macrophages in the zebrafish embryo. Development. 1999;126:3735–3745
  7. Burns CE, DeBlasio T, Zhou Y, Zhang J, Zon L, Nimer SD. Isolation and characterization of runxa and runxb, zebrafish members of the runt family of transcriptional regulators. Exp Hematol. 2002;30:1381–1389
  8. Hsia N, Zon LI. Transcriptional regulation of hematopoietic stem cell development in zebrafish. Exp Hematol. 2005;33:1007–1014
  9. Rane SG, Reffy EP. JAKs, STATs and Src kinases in hematopoiesis. Oncogene. 2002;21:3334–3358
  10. Ward AC, Touw I, Yoshimura A. The Jak-Stat pathway in normal and perturbed hematopoiesis. Blood. 2000;95:19–29
  11. Oates AC, Brownlie A, Pratt SJ, et al. Gene Duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis. Blood. 1999;94:2622–2636
  12. Ma AC, Ward AC, Liang R, Leung AY. The role of jak2a in zebrafish hematopoiesis. Blood. 2007;110:1824–1830
  13. James C, Ugo V, Le Couedic JP, et al. A unique clonal JAK2 mutation leading to constitutive signaling causes polycythaemia vera. Nature. 2005;434(7037):1144–1148
  14. Kralovics R, Passamonti F, Buser AS, et al. A gain-of-function mutation of JAK2 in myeloproliferative disorders. N Engl J Med. 2005;352:1779–1790
  15. Baxter EJ, Scott LM, Campbell PJ, et al. Cancer Genome Project. Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders. Lancet. 2005;365(9464):1054–1061
  16. Xing S, Wanting TH, Zhao W, et al. Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice. Blood. 2008;111:5109–5117
  17. Shide K, Shimoda HK, Kumano T, et al. Development of ET, primary myelofibrosis and PV in mice expressing JAK2 V617F. Leukemia. 2008;22:87–95
  18. Tiedt R, Hao-Shen H, Sobas MA, et al. Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood. 2008;111:3931–3940
  19. Lacout C, Pisani DF, Tulliez M, Gachelin FM, Vainchenker W, Villeval JL. JAK2V617F expression in murine hematopoietic cells leads to MPD mimicking human PV with secondary myelofibrosis. Blood. 2006;108:1652–1660
  20. Wernig G, Mercher T, Okabe R, Levine RL, Lee BH, Gilliland DG. Expression of Jak2V617F causes a polycythemia vera-like disease with associated myelofibrosis in a murine bone marrow transplant model. Blood. 2006;107:4274–4281
  21. Bumm TG, Elsea C, Corbin AS, et al. Characterization of murine JAK2V617F-positive myeloproliferative disease. Cancer Res. 2006;66:11156–11165
  22. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. Stages of embryonic development of the zebrafish. Dev Dyn. 1995;203:253–310
  23. Long Q, Meng A, Wang H, Jessen JR, Farrell MJ, Lin S. GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development. 1997;124:4105–4111
  24. Leung AY, Mendenhall EM, Kwan TT, et al. Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos. Dev Biol. 2005;277:235–254
  25. Lewis RS, Stephenson SE, Ward AC. Constitutive activation of zebrafish Stat5 expands hematopoietic cell populations in vivo. Exp Hematol. 2006;34:179–187
  26. Chu CY, Cheng CH, Chen GD, et al. The zebrafish erythropoietin: functional identification and biochemical identification. FEBS Lett. 2007;581:4265–4271
  27. Lai JC, Kakuta I, Mok HO, Rummer JL, Randall D. Effects of moderate and substantial hypoxia on erythropoietin levels in rainbow trout kidney and spleen. J Exp Biol. 2006;209:2734–2738
  28. Paffett-Lugassy N, Hsia N, Fraenkel PG, et al. Functional conservation of erythropoietin signaling in zebrafish. Blood. 2007;110:2718–2726
  29. Luo H, Dearolf CR. The JAK/STAT pathway and Drosophila development. Bioessays. 2001;23:1138–1147
  30. Pardanani A, Hood J, Lasho T, et al. TG101209, a small molecule JAK2-selective kinase inhibitor potently inhibits myeloproliferative disorder-associated JAK2V617F and MPLW515L/K mutations. Leukemia. 2007;21:1658–1668
  31. Manshouri T, Quintas-Cardama A, Nussenzveig RH, et al. The JAK kinase inhibitor CP-690,550 suppresses the growth of human polycythemia vera cell carrying the JAK2V617F mutation. Cancer Sci. 2008;99:1265–1273
  32. Werning G, Kharas MG, Okabe R, et al. Efficacy of TG101348, a selective JAK2 inhibitor, in treatment of a murine model of JAK2V617F-induced polycythemia vera. Cancer Cell. 2008;13:311–320

PII: S0301-472X(09)00367-1

doi: 10.1016/j.exphem.2009.08.008

Experimental Hematology
Volume 37, Issue 12 , Pages 1379-1386.e4 , December 2009

Article Tools

* Image Usage & Resolution