Experimental Hematology
Volume 37, Issue 8 , Pages 887-888 , August 2009

Nanon effects on mammalian cell long-term cultures: A caveat for experimental hematologists dealing with in vitro long-term culture assays

  • Peppino Mirabelli

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • ,
  • Francesca D'Alessio

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • ,
  • Elisabetta Mariotti

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • ,
  • Maria Romano

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • ,
  • Giuliana Fortunato

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • ,
  • Marica Gemei

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • European School of Molecular Medicine, Napoli, Italy
    • ,
  • Rosa Di Noto

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy
    • Corresponding Author InformationOffprint requests to: Rosa Di Noto, B.Sc., CEINGE, Biotecnologie Avanzate, Via Comunale Margherita 482, 80145 Napoli, Italy
    • ,
  • Luigi Del Vecchio

      Affiliations

    • CEINGE, Biotecnologie Avanzate, Napoli, Italy
    • Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Napoli, Italy

References 

  1. Kajander EO, Çiftçioglu N. Nanobacteria: an alternative mechanism for pathogenetic intra- and extracellular calcification and stone formation. Proc Natl Acad Sci U S A. 1998;95:8274–8279
  2. Kajander EO, Çiftçioglu N, Aho K, Garcia-Cuerpo E. Characteristics of nanobacteria and their possible role in stone formation. Urol Res. 2003;31:47–54
  3. Simonetti AB, Englert GE, Campos K, et al. Nanobacteria-like particles: a threat to cell cultures. Braz J Microbiol. 2007;38:153–158
  4. Raoult D, Drancourt M, Azza S, et al. Nanobacteria are mineralo fetuin complexes. PLoS Pathog. 2008;4:e41
  5. Urbano P, Urbano F. Nanobacteria: facts or fancies?. PLoS Pathog. 2007;35:e55
  6. United Kingdom Coordinating Committee on Cancer Research guidelines for the use of cell lines in cancer research. Br J Cancer. 2000;82:1495–1509
  7. Di Noto R, Mirabelli P, Mariotti E, et al. CMRL-T, a novel T-cell line showing asynchronous phenotype (CD34+/CD1a-/TCRαβ+) and dual T-cell receptor β chain. Leukemia. 2006;20:2175–2177
  8. Di Noto R, Pane F, Camera A, et al. Characterization of two novel cell lines, DERL-2 (CD56+/CD3+/TCRγδ+) and DERL-7 (CD56+/CD3-/TCRγ-δ-), derived from a single patient with CD56+ non-Hodgkin's lymphoma. Leukemia. 2001;15:1641–1649
  9. Bass H, Walters R, Darke C. Provision of Epstein-Barr virus-transformed B-cell lines in a routine tissue typing laboratory: practicalities and applications. Eur J Immunogenet. 2004;31:87–92
  10. Manor E. Human plasma accelerates immortalization of B lymphocytes by Epstein-Barr virus. Cell Prolif. 2008;41:292–298

PII: S0301-472X(09)00210-0

doi: 10.1016/j.exphem.2009.05.013

Experimental Hematology
Volume 37, Issue 8 , Pages 887-888 , August 2009

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