Experimental Hematology
Volume 37, Issue 9 , Pages 1084-1095 , September 2009

Cord blood stem cell expansion is permissive to epigenetic regulation and environmental cues

  • Hiroto Araki

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Sudhakar Baluchamy

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • Dr. Baluchamy and Ms. Yoshinaga contributed equally to this work.
    • ,
  • Kazumi Yoshinaga

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • Dr. Baluchamy and Ms. Yoshinaga contributed equally to this work.
    • ,
  • Benjamin Petro

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Sakina Petiwala

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Ritesh Parajuli

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Mohammed Milhem

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Donald Lavelle

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Joseph DeSimone

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • ,
  • Nadim Mahmud

      Affiliations

    • Section of Hematology/Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Ill., USA
    • University of Illinois Cancer Center, Chicago, Ill., USA
    • Corresponding Author InformationOffprint requests to: Nadim Mahmud, M.D., Ph.D., University of Illinois at Chicago, 909 South Wolcott Avenue, COMRB, Room #3095, Chicago, IL 60607

Received 3 March 2009 ,Revised 15 May 2009 ,Accepted 28 May 2009.

References 

  1. Blau HM. Differentiation requires continuous active control. Annu Dev Biochem. 1992;61:1213–1230
  2. Jones PA, Takai D. The role of DNA methylation in mammalian epigenetics. Science. 2001;293:1068–1070
  3. Marks PA, Richon VM, Rifkind RA. Histone deacetylases inhibitors: inducers of differentiation or apoptosis of transformed cells. J Natl Cancer Inst. 2000;92:1210–1216
  4. Kass SU, Pruss D, Wolffe AP. How dose methylation mediated repress transcription?. Trends Genet. 1997;13:444–449
  5. Issa JP. Decitabine. Curr Opin Oncol. 2003;15:446–451
  6. Mahmud N, Milhem M, Araki H, Hoffman R. Alteration of hematopoietic stem cell fates by chromatin-modifying agents. In:  Ho AD,  Hoffman R,  Zanjani ED editor. Frontiers in Stem Cell Transplantation. Weinheim, Germany: Wiley-VCH; 2006;p. 27–42
  7. Ueda T, Tsuji K, Yoshino H, et al. Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor. J Clin Invest. 2000;105:1013–1021
  8. Piacibello W, Sanavio F, Severino A, et al. Engraftment in nonobese diabetic severe combined immunodeficient mice of human CD34+ cord blood cells after ex vivo expansion: evidence for the amplification and self-renewal of repopulating stem cells. Blood. 1999;93:3736–3749
  9. Habibian HK, Peters SO, Hsieh CC, et al. The fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit. J Exp Med. 1998;188:393–398
  10. Takatoku M, Sellers S, Agricola BA, et al. Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates. J Clin Invest. 2001;108:447–455
  11. Glimm H, Oh IH, Eaves CJ. Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G(2)/M transit and do not reenter G(0). Blood. 2000;96:4185–4193
  12. Jaenisch R, Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat Gene. 2003;33:245–254
  13. Araki H, Yoshinaga K, Boccuni P, Zhao Y, Hoffman R, Mahmud N. Chromatin modifying agents permit human hematopoietic stem cells to undergo multiple cell divisions while retaining their repopulating potential. Blood. 2007;109:3570–3578
  14. Araki H, Mahmud N, Milhem M, et al. Expansion of human umbilical cord blood SCID-repopulating cells using chromatin modifying agents. Exp Hematol. 2006;34:140–149
  15. Xu M, Bruno E, Chao J, et al. MPD research consortium. Constitutive mobilization of CD34+ cells into the peripheral blood in idiopathic myelofibrosis may be due to the action of a number of proteases. Blood. 2005;105:4508–4515
  16. Cameron EE, Bachman KE, Myöhänen S, Herman JG, Baylin SB. Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nat Genet. 1999;21:103–107
  17. Momparler RL. Pharmacology of 5-Aza-2′-deoxycytidine (decitabine). Semin Hematol. 2005;42:9–16
  18. Dercksen MW, Gerritsen WR, Rodenhuis S, et al. Expression of adhesion molecules on CD34+ cells: CD34 + L-selectin+ cells predict a rapid platelet recovery after peripheral blood stem cell transplantation. Blood. 1995;85:3313–3319
  19. Peled A, Petit I, Kollet O, et al. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science. 1999;283:845–848
  20. Christopherson KW, Hangoc G, Mantel CR, Broxmeyer HE. Modulation of hematopoietic stem cell homing and engraftment by CD26. Science. 2004;305:1000–1003
  21. Devine SM, Lazarus HM, Emerson SG. Clinical application of hematopoietic progenitor cell expansion: current status and future prospects. Bone Marrow Transplant. 2003;31:241–252
  22. Goff JP, Shields DS, Greenberger JS. Influence of cytokines on the growth kinetics and immunophenotype of daughter cells resulting from the first division of single CD34 + Thy+lin- cells. Blood. 1998;92:4098–4107
  23. Cheng T, Rodrigues N, Shen H, et al. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science. 2000;287:1804–1808
  24. Stier S, Cheng T, Forkert R, et al. Ex vivo targeting of p21Cip1/Waf1 permits relative expansion of human hematopoietic stem cells. Blood. 2003;102:1260–1266
  25. Mahmud N, Katayama N, Itoh R, Tanaka R, Ohishi K, Masuya M, et al. A possible change in doubling time of haemopoietic progenitor cells with stem cell development. Br J Haematol. 1996;94:242–249
  26. Mahmud N, Devine SM, Weller KP, et al. The relative quiescence of hematopoietic stem cells in nonhuman primates. Blood. 2001;97:3061–3068
  27. Ho AD. Kinetics and symmetry of divisions of hematopoietic stem cells. Exp Hematol. 2005;33:1–8
  28. Srour EF. Proliferative history and hematopoietic function of ex vivo expanded human CD34+ cells. Blood. 2000;96:1609–1612
  29. Young JC, Lin K, Hansteen G, et al. CD34+ cells from mobilized peripheral blood retain fetal bone marrow repopulating capacity within the Thy-1+ subset following cell division ex vivo. Exp Hematol. 1999;27:994–1003
  30. Zandstra PW, Conneally E, Petzer AL, Piret JM, Eaves CJ. Cytokine manipulation of primitive human hematopoietic cell self-renewal. Proc Natl Acad Sci U S A. 1997;94:4698–4703
  31. Prosper F, Stroncek D, McCarthy JB, Verfaillie CM. Mobilization and homing of peripheral blood progenitors is related to reversible downregulation of α4β1 integrin expression and function. J Clin Invest. 1998;101:2456–2467
  32. Foguenne J, Di Stefano I, Giet O, Beguin Y, Gothot A. Ex vivo expansion of hematopoietic progenitor cells is associated with downregulation of alpha 4 integrin and CXCR4-mediated engraftment in NOD/SCID beta2-microglobulin-null mice. Haematologica. 2009;94:185–194

PII: S0301-472X(09)00209-4

doi: 10.1016/j.exphem.2009.05.012

Experimental Hematology
Volume 37, Issue 9 , Pages 1084-1095 , September 2009

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