Experimental Hematology
Volume 37, Issue 6 , Pages 728-738 , June 2009

Comprehensive assessment of the TCRBV repertoire in small T-cell samples by means of an improved and convenient multiplex PCR method

  • Sara Mariani

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
  • ,
  • Seung Yae Hwang

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
  • ,
  • Myriam Foglietta

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
  • ,
  • Lisa Bonello

      Affiliations

    • Dipartimento di Scienze Biomediche ed Oncologia Umana, Università di Torino, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
  • ,
  • Candida Vitale

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
  • ,
  • Marta Coscia

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
  • ,
  • Francesca Fiore

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy
    • Corresponding Author InformationOffprint requests to: Francesca Fiore, M.D., Divisione di Ematologia dell'Università di Torino, Via Genova 3, 10126 Torino, Italy
  • ,
  • Benedetto Bruno

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
  • ,
  • Massimo Massaia

      Affiliations

    • Divisione di Ematologia dell'Università di Torino, Torino, Italy
    • Laboratorio di Ematologia Oncologia, Centro di Ricerca in Medicina Sperimentale (CeRMS), Ospedale San Giovanni Battista, Torino, Italy

Received 17 January 2009 ,Revised 6 March 2009 ,Accepted 9 March 2009.

References 

  1. Nikolich-Zugich J, Slifka MK, Messaoudi I. The many important facets of T-cell repertoire diversity. Nat Rev Immunol. 2004;4:123–132
  2. Kedzierska K, La Gruta NL, Stambas J, Turner SJ, Doherty PC. Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity. Mol Immunol. 2008;45:607–618
  3. McCloskey TW, Haridas V, Pahwa R, Pahwa S. T cell receptor V beta repertoire of the antigen specific CD8 T lymphocyte subset of HIV infected children. Aids. 2002;16:1459–1465
  4. Quaratino S, Feldmann M, Dayan CM, Acuto O, Londei M. Human self-reactive T cell clones expressing identical T cell receptor beta chains differ in their ability to recognize a cryptic self-epitope. J Exp Med. 1996;183:349–358
  5. Alvarez CM, Opelz G, Giraldo MC, et al. Evaluation of T-cell receptor repertoires in patients with long-term renal allograft survival. Am J Transplant. 2005;5:746–756
  6. Hu H, Queiro MR, Tilanus MG, de Weger RA, Schuurman HJ. Expression of T-cell receptor alpha and beta variable genes in normal and malignant human T cells. Br J Haematol. 1993;84:39–48
  7. Gorski J, Yassai M, Zhu X, et al. Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping. Correlation with immune status. J Immunol. 1994;152:5109–5119
  8. Pannetier C, Even J, Kourilsky P. T-cell repertoire diversity and clonal expansions in normal and clinical samples. Immunol Today. 1995;16:176–181
  9. Maslanka K, Piatek T, Gorski J, Yassai M, Gorski J. Molecular analysis of T cell repertoires. Spectratypes generated by multiplex polymerase chain reaction and evaluated by radioactivity or fluorescence. Hum Immunol. 1995;44:28–34
  10. Fernandes S, Chavan S, Chitnis V, Kohn N, Pahwa S. Simplified fluorescent multiplex PCR method for evaluation of the T-cell receptor V beta-chain repertoire. Clin Diagn Lab Immunol. 2005;12:477–483
  11. Akatsuka Y, Martin EG, Madonik A, Barsoukov AA, Hansen JA. Rapid screening of T-cell receptor (TCR) variable gene usage by multiplex PCR: application for assessment of clonal composition. Tissue Antigens. 1999;53:122–134
  12. van Dongen JJ, Langerak AW, Bruggemann M, et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2003;17:2257–2317
  13. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156–159
  14. Mariani S, Coscia M, Even J, et al. Severe and long-lasting disruption of T-cell receptor diversity in human myeloma after high-dose chemotherapy and autologous peripheral blood progenitor cell infusion. Br J Haematol. 2001;113:1051–1059
  15. Puisieux I, Even J, Pannetier C, Jotereau F, Favrot M, Kourilsky P. Oligoclonality of tumor-infiltrating lymphocytes from human melanomas. J Immunol. 1994;153:2807–2818
  16. Halle JP, Haus-Seuffert P, Woltering C, Stelzer G, Meisterernst M. A conserved tissue-specific structure at a human T-cell receptor beta-chain core promoter. Mol Cell Biol. 1997;17:4220–4229
  17. Kuniyasu Y, Takahashi T, Itoh M, Shimizu J, Toda G, Sakaguchi S. Naturally anergic and suppressive CD25 + CD4 + T cells as a functionally and phenotypically distinct immunoregulatory T cell subpopulation. Int Immunol. 2000;12:1145–1155
  18. Fujishima M, Hirokawa M, Fujishima N, Sawada K. TCRalphabeta repertoire diversity of human naturally occurring CD4 + CD25+ regulatory T cells. Immunol Lett. 2005;99:193–197
  19. Chen X, Hale GA, Neale GA, et al. A novel approach for the analysis of T-cell reconstitution by using a T-cell receptor beta-based oligonucleotide microarray in hematopoietic stem cell transplantation. Exp Hematol. 2007;35:831–841
  20. Halapi E, Werner A, Wahlstrom J, et al. T cell repertoire in patients with multiple myeloma and monoclonal gammopathy of undetermined significance: clonal CD8 + T cell expansions are found preferentially in patients with a low tumor burden. Eur J Immunol. 1997;27:2245–2252
  21. Plasilova M, Risitano A, Maciejewski JP. Application of the molecular analysis of the T-cell receptor repertoire in the study of immune-mediated hematologic diseases. Hematology. 2003;8:173–181
  22. Kedzierska K, Venturi V, Field K, Davenport MP, Turner SJ, Doherty PC. Early establishment of diverse T cell receptor profiles for influenza-specific CD8(+)CD62L(hi) memory T cells. Proc Natl Acad Sci U S A. 2006;103:9184–9189
  23. Venturi V, Kedzierska K, Tanaka MM, Turner SJ, Doherty PC, Davenport MP. Method for assessing the similarity between subsets of the T cell receptor repertoire. J Immunol Methods. 2008;329:67–80
  24. Ahmadian A, Ehn M, Hober S. Pyrosequencing: history, biochemistry and future. Clin Chim Acta. 2006;363:83–94
  25. Brusko TM, Putnam AL, Bluestone JA. Human regulatory T cells: role in autoimmune disease and therapeutic opportunities. Immunol Rev. 2008;223:371–390
  26. Kasow KA, Chen X, Knowles J, Wichlan D, Handgretinger R, Riberdy JM. Human CD4 + CD25+ regulatory T cells share equally complex and comparable repertoires with CD4 + CD25- counterparts. J Immunol. 2004;172:6123–6128
  27. Ou Y, Tong C, Zhang Y, et al. An improved design of PCR primers for detection of human T cell receptor β chain repertoire. Mol Biol Rep. 2009;36:145–152
  28. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40:27–36

PII: S0301-472X(09)00081-2

doi: 10.1016/j.exphem.2009.03.003

Experimental Hematology
Volume 37, Issue 6 , Pages 728-738 , June 2009