Experimental Hematology
Volume 36, Issue 12 , Pages 1648-1659 , December 2008

Triptolide cooperates with chemotherapy to induce apoptosis in acute myeloid leukemia cells

  • Arnaud Pigneux

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • INSERM, E876, Bordeaux, France
    • Univ V Segalen cCHU Bordeaux, Hôpital Haut-Lévêque, Service des Maladies du Sang, Pessac, France
    • Corresponding Author InformationOffprint requests to: Arnaud Pigneux, M.D., Ph.D., Service des Maladies du Sang, Hôpital Haut-Lévêque, Avenue de Magellan, 33604 Pessac, France
    • ,
  • François-Xavier Mahon

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • INSERM, E876, Bordeaux, France
    • ,
  • Maialene Uhalde

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • ,
  • Marie Jeanneteau

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • ,
  • Francis Lacombe

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • ,
  • Noël Milpied

      Affiliations

    • Univ V Segalen cCHU Bordeaux, Hôpital Haut-Lévêque, Service des Maladies du Sang, Pessac, France
    • ,
  • Josy Reiffers

      Affiliations

    • Institut Bergonié, Bordeaux, France
    • ,
  • Francis Belloc

      Affiliations

    • CHU Bordeaux, Hôpital Haut-Lévêque, Laboratoire d'Hématologie, Pessac, France
    • INSERM, E876, Bordeaux, France

Received 14 April 2008 ,Revised 25 July 2008 ,Accepted 6 August 2008.

References 

  1. Lowenberg B, Downing JR, Burnett A. Acute myeloid leukemia. N Engl J Med. 1999;341:1051–1062
  2. Weltermann A, Fonatsch C, Haas OA, et al. Impact of cytogenetics on the prognosis of adults with de novo AML in first relapse. Leukemia. 2004;18:293–302
  3. Jiang X. Clinical observations on the use of the Chinese herb Tripterygium wilfordii Hook for the treatment of nephrotic syndrome. Pediatr Nephrol. 1994;8:343–344
  4. Tao XL, Sun Y, Dong Y, et al. A prospective, controlled, double-blind, cross-over study of tripterygium wilfodii hook F in treatment of rheumatoid arthritis. Chin Med J (Engl). 1989;102:327–332
  5. Kupchan SM, Court WA, Dailey RG, Gilmore CJ, Bryan RF. Triptolide and tripdiolide, novel antileukemic diterpenoid triepoxides from Tripterygium wilfordii. J Am Chem Soc. 1972;94:7194–7195
  6. Yang Y, Liu Z, Tolosa E, Yang J, Li L. Triptolide induces apoptotic death of T lymphocyte. Immunopharmacology. 1998;40:139–149
  7. Shamon LA, Pezzuto JM, Graves JM, et al. Evaluation of the mutagenic, cytotoxic, and antitumor potential of triptolide, a highly oxygenated diterpene isolated from Tripterygium wilfordii. Cancer Lett. 1997;112:113–117
  8. Chen BJ. Triptolide, a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F. Leuk Lymphoma. 2001;42:253–265
  9. Fidler JM, Li K, Chung C, et al. PG490-88, a derivative of triptolide, causes tumor regression and sensitizes tumors to chemotherapy. Mol Cancer Ther. 2003;2:855–862
  10. Yang S, Chen J, Guo Z, et al. Triptolide inhibits the growth and metastasis of solid tumors. Mol Cancer Ther. 2003;2:65–72
  11. Kiviharju TM, Lecane PS, Sellers RG, Peehl DM. Antiproliferative and proapoptotic activities of triptolide (PG490), a natural product entering clinical trials, on primary cultures of human prostatic epithelial cells. Clin Cancer Res. 2002;8:2666–2674
  12. Pu LX, Zhang TM. [Effects of triptolide on T lymphocyte functions in mice]. Zhongguo Yao Li Xue Bao. 1990;11:76–79
  13. Chan EW, Cheng SC, Sin FW, Xie Y. Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines. Toxicol Lett. 2001;122:81–87
  14. Choi YJ, Kim TG, Kim YH, et al. Immunosuppressant PG490 (triptolide) induces apoptosis through the activation of caspase-3 and down-regulation of XIAP in U937 cells. Biochem Pharmacol. 2003;66:273–280
  15. Carter BZ, Mak DH, Schober WD, et al. Triptolide induces caspase-dependent cell death mediated via the mitochondrial pathway in leukemic cells. Blood. 2006;108:630–637
  16. Frese S, Pirnia F, Miescher D, et al. PG490-mediated sensitization of lung cancer cells to Apo2L/TRAIL-induced apoptosis requires activation of ERK2. Oncogene. 2003;22:5427–5435
  17. Lee KY, Chang W, Qiu D, Kao PN, Rosen GD. PG490 (triptolide) cooperates with tumor necrosis factor-alpha to induce apoptosis in tumor cells. J Biol Chem. 1999;274:13451–13455
  18. Chang WT, Kang JJ, Lee KY, et al. Triptolide and chemotherapy cooperate in tumor cell apoptosis. A role for the p53 pathway. J Biol Chem. 2001;276:2221–2227
  19. Zhao G, Vaszar LT, Qiu D, Shi L, Kao PN. Anti-inflammatory effects of triptolide in human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2000;279:L958–L966
  20. Qiu D, Zhao G, Aoki Y, et al. Immunosuppressant PG490 (triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-kappaB transcriptional activation. J Biol Chem. 1999;274:13443–13450
  21. Liu Q, Chen T, Chen H, et al. Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 MAP kinase phosphorylation and caspase 3 activation. Biochem Biophys Res Commun. 2004;319:980–986
  22. Odero MD, Zeleznik-Le NJ, Chinwalla V, Rowley JD. Cytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer. 2000;29:333–338
  23. Lacombe F, Durrieu F, Briais A, et al. Flow cytometry CD45 gating for immunophenotyping of acute myeloid leukemia. Leukemia. 1997;11:1878–1886
  24. Vial JP, Tabrizi R, Pigneux A, Lacombe F, Praloran V, Belloc F. Remission induction chemotherapy induces in vivo caspase-dependent apoptosis in bone marrow acute myeloid leukemia blast cells and spares lymphocytes. Cytometry B Clin Cytom. 2006;70:115–123
  25. Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994;84:1415–1420
  26. Vayssiere JL, Petit PX, Risler Y, Mignotte B. Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40. Proc Natl Acad Sci U S A. 1994;91(24):11752–11756
  27. Tao D, Wu J, Feng Y, Qin J, Hu J, Gong J. New method for the analysis of cell cycle-specific apoptosis. Cytometry A. 2004;57:70–74
  28. Belloc F, Belaud-Rotureau MA, Lavignolle V, et al. Flow cytometry detection of caspase 3 activation in preapoptotic leukemic cells. Cytometry. 2000;40:151–160
  29. Lacombe F, Belloc F, Dumain P, et al. Quantitation of resistance of leukemic cells to cytosine arabinoside from BrdUrd/DNA bivariate histograms. Cytometry. 1992;13:730–738
  30. Lacombe F, Belloc F, Dumain P, et al. Detection of cytarabine resistance in patients with acute myelogenous leukemia using flow cytometry. Blood. 1994;84:716–723
  31. Chou TC, Talalay P. Generalized equations for the analysis of inhibitions of Michaelis-Menten and higher-order kinetic systems with two or more mutually exclusive and nonexclusive inhibitors. Eur J Biochem. 1981;115:207–216
  32. Lee KY, Park JS, Jee YK, Rosen GD. Triptolide sensitizes lung cancer cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis by inhibition of NF-kappaB activation. Exp Mol Med. 2002;34:462–468
  33. Carter BZ, Mak DH, Schober WD, et al. Triptolide sensitizes AML cells to TRAIL-induced apoptosis via decrease of XIAP and p53-mediated increase of DR5. Blood. 2008;111:3742–3750
  34. Freedman DA, Wu L, Levine AJ. Functions of the MDM2 oncoprotein. Cell Mol Life Sci. 1999;55:96–107
  35. Vial JP, Belloc F, Dumain P, et al. Study of the apoptosis induced in vitro by antitumoral drugs on leukaemic cells. Leuk Res. 1997;21:163–172

PII: S0301-472X(08)00387-1

doi: 10.1016/j.exphem.2008.08.002

Experimental Hematology
Volume 36, Issue 12 , Pages 1648-1659 , December 2008

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