Experimental Hematology
Volume 35, Issue 12 , Pages 1777-1781, December 2007

Telomere length in paroxysmal nocturnal hemoglobinuria correlates with clone size

  • Gabriela M. Baerlocher

      Affiliations

    • Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
    • Department of Hematology, University of Bern, Bern, Switzerland
    • ,
  • Elaine M. Sloand

      Affiliations

    • Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    • ,
  • Neal S. Young

      Affiliations

    • Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    • ,
  • Peter M. Lansdorp

      Affiliations

    • Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
    • Department of Medicine, University of British Columbia, Vancouver, BC, Canada
    • Corresponding Author InformationOffprint requests to: Peter M. Lansdorp, M.D., Ph.D., Terry Fox Laboratory, 675 West 10th Avenue, 11th Floor, Vancouver, BC, V5Z 1L3, Canada

Received 27 March 2007; received in revised form 5 June 2007; accepted 6 June 2007. published online 15 August 2007.

Objective

To study if telomere length can be used as a surrogate marker for the mitotic history in normal and affected hematopoietic cells from patients with paroxysmal nocturnal hemoglobinuria (PNH).

Methods

The telomere length was measured by automated multicolor flow fluorescence in situ hybridization in glycosyl-phosphatidyl-inositol anchored protein (GPI)–negative and GPI-positive peripheral blood leukocytes. Eleven patients were studied, two with predominantly hemolytic PNH and nine with PNH associated with marrow failure.

Results

Telomere length in GPI-negative cells was significantly shorter than in GPI-positive cells of the same patient (p < 0.01, n = 11). The difference in telomere length (telomere length in GPI-positive minus telomere length in GPI-negative cells) correlated with the percentage of GPI-negative white blood cells.

Conclusion

Our results support the hypothesis that telomere length is correlated to the replicative history of GPI-positive and GPI-negative cells and warrant further studies of telomere length in relation to disease progression in PNH.

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PII: S0301-472X(07)00381-5

doi:10.1016/j.exphem.2007.06.010

Experimental Hematology
Volume 35, Issue 12 , Pages 1777-1781, December 2007

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