Experimental Hematology
Volume 34, Issue 10 , Pages 1353-1359 , October 2006

A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system

  • Raquel Gonçalves

      Affiliations

    • Department of Animal Biotechnology, University of Nevada, Reno, Nev., USA
    • Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Lisboa, Portugal
    • These authors contributed equally to this work.
    • ,
  • Cláudia Lobato da Silva

      Affiliations

    • Department of Animal Biotechnology, University of Nevada, Reno, Nev., USA
    • Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Lisboa, Portugal
    • These authors contributed equally to this work.
    • ,
  • Joaquim M.S. Cabral

      Affiliations

    • Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Lisboa, Portugal
    • ,
  • Esmail D. Zanjani

      Affiliations

    • Department of Animal Biotechnology, University of Nevada, Reno, Nev., USA
    • ,
  • Graça Almeida-Porada

      Affiliations

    • Department of Animal Biotechnology, University of Nevada, Reno, Nev., USA
    • Corresponding Author InformationOffprint requests to: Graça Almeida-Porada, M.D., Ph.D., Department of Animal Biotechnology, University of Nevada, Reno, Mail Stop 202, Reno NV 89557-0104

Received 15 May 2006 ,Accepted 15 May 2006.

References 

  1. Almeida-Porada G, Brown R, Mackintosh FR, et al. Evaluation of serum free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. J Hematother Stem Cell Res. 2000;5:683–693
  2. Emerson SG. Ex vivo expansion of hematopoietic precursors, progenitors, and stem cells: the next generation of cellular therapeutics. Blood. 1996;87:3082–3088
  3. Lobato da Silva C, Gonçalves R, Crapnell KB, et al. A human stromal-based serum free culture system supports the ex-vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells. Exp Hematol. 2005;33:828–835
  4. Douay L. Experimental culture conditions are critical for ex vivo expansion of hematopoietic cells. J Hematother Stem Cell Res. 2001;10:341–346
  5. Brandt JE, Bartholomew AM, Fortman JD, et al. Ex vivo expansion of autologous bone marrow CD34(+) cells with porcine microvascular endothelial cells results in a graft capable of rescuing lethally irradiated baboons. Blood. 1999;94:106–113
  6. Shih CC, Hu MC, Hu J, Medeiros J, et al. Long-term ex vivo maintenance and expansion of transplantable human hematopoietic stem cells. Blood. 1999;94:1623–1636
  7. Breems DA, Blokland EA, Siebel KE, et al. Stroma-contact prevents loss of hematopoietic stem cell quality during ex vivo expansion of CD34+ mobilized peripheral blood stem cells. Blood. 1998;91:111–117
  8. Petzer AL, Hogge DE, Landsdorp PM, et al. Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium. Proc Natl Acad Sci U S A. 1996;93:1470–1474
  9. Chute JP, Saini AA, Chute DJ, et al. Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow. Blood. 2002;100:4433–4439
  10. Gartner S, Kaplan HS. Long-term culture of human bone marrow cells. Proc Natl Acad Sci U S A. 1980;77:4756–4759
  11. Simmons PJ, Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood. 1991;78:55–62
  12. Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol. 1998;176:57–66
  13. Dennis JE, Charbord P. Origin and differentiation of human and murine stroma. Stem Cells. 2002;20:205–214
  14. Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol. 2000;28:875–884
  15. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Bio. 2004;36:568–584
  16. Dexter TM, Moore MA, Sheridan AP. Maintenance of hemopoietic stem cells and production of differentiated progeny in allogeneic and semiallogeneic bone marrow chimerasin vitro. J Exp Med. 1997;145:1612–1626
  17. Moore KA, Ema H, Lemischka IR. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood. 1997;89:4337–4347
  18. Koschmieder S, Bug G, Schroder B, et al. Murine M2-10B4 and SL/SL cell lines differentially affect the balance between CD34+ cell expansion and maturation. Int J Hematol. 2001;73:71–77
  19. Bennaceur-Griscelli A, Pondarre C, Schiavon V, et al. Stromal cells retard the differentiation of CD34(+)CD38(low/neg) human primitive progenitors exposed to cytokines independent of their mitotic history. Blood. 2001;97:435–441
  20. Knospe WH, Husseini SG, Zipori D, et al. Hematopoiesis on cellulose ester membranes. XIII. A combination of cloned stromal cells is needed to establish a hematopoietic microenvironment supportive of trilineal hematopoiesis. Exp Hematol. 1993;21:257–262
  21. De Angeli S, Di Liddo R, Buoro S, et al. New immortalized human stromal cell lines enhancing in vitro expansion of cord blood hematopoietic stem cells. Int J Mol Med. 2004;13:363–371
  22. McNiece I, Harrington J, Turney J, et al. Ex vivo expansion of cord blood mononuclear cells on mesenchymal stem cells. Cytotherapy. 2004;6:311–317
  23. Almeida-Porada G, Porada CD, Tran N, Zanjani ED. Cotransplantation of human stromal cell progenitors into preimmune fetal sheep results in early appearance of human donor cells in circulation and boosts cell levels in bone marrow at later time points after transplantation. Blood. 2000;95:3620–3627
  24. Loeuillet C, Bernard G, Remy-Martin J, et al. Distinct hematopoietic support by two human stromal cell lines. Exp Hematol. 2001;29:736–745
  25. Gammaitoni L, Bruno S, Sanavio F, et al. Ex vivo expansion of human adult stem cells capable of primary and secondary hemopoietic reconstitution. Exp Hematol. 2003;31:261–270
  26. Shimakura Y, Kawada H, Ando K, et al. Murine stromal cell line HESS-5 maintains reconstituting ability of ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood. Stem Cells. 2000;18:183–189
  27. Kawada H, Ando K, Tsuji T, et al. Rapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system. Exp Hematol. 1999;27:904–915
  28. Frias AM, Porada C, Cabral JMS, et al. Development of a novel culture system that allows that allows both lymphoid and myeloid development from human umbilical cord blood. Exp Hematol. 2004;32:32a
  29. Yao CL, Chu IM, Hsieh TB, et al. A systematic strategy to optimize ex vivo expansion medium for human hematopoietic stem cells derived from umbilical cord blood mononuclear cells. Exp Hematol. 2004;32:720–727
  30. Lewis ID, Almeida-Porada G, Du J, et al. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood. 2001;97:3441–3449
  31. McNiece IK, Almeida-Porada G, Shpall EJ, et al. Ex vivo expanded cord blood cells provide rapid engraftment in fetal sheep but lack long-term engrafting potential. Exp Hematol. 2002;30:612–616

PII: S0301-472X(06)00496-6

doi: 10.1016/j.exphem.2006.05.024

Experimental Hematology
Volume 34, Issue 10 , Pages 1353-1359 , October 2006

Article Tools

* Image Usage & Resolution