Experimental Hematology
Volume 34, Issue 11 , Pages 1596-1601, November 2006

Multipotent adult progenitor cell isolation and culture procedures

University of Minnesota, Stem Cell Institute, Minneapolis, Minn., USA

Received 30 January 2006; received in revised form 30 January 2006; accepted 14 July 2006.

We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.

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PII: S0301-472X(06)00453-X

doi:10.1016/j.exphem.2006.07.013

Experimental Hematology
Volume 34, Issue 11 , Pages 1596-1601, November 2006

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